This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, W. Articles by Marks, J. R. Search for Related Content PubMed PubMed Citation Articles by Wang, W. Articles by Marks, J. R.

---

Cell Cycle, Cell Death, and Senescence

TAF_II70 Isoform-Specific Growth Suppression Correlates With Its Ability to Complex With the GADD45a Protein¹

Department of Surgery, Duke University Medical Center, Durham, North Carolina

Requests for reprints: Jeffrey R. Marks, Department of Surgery, Duke University Medical Center, Box 3873, Durham, NC 27710. Phone: 919-681-6133; Fax: 919-681-6291. E-mail: marks003{at}mc.duke.edu

TAF_II70, a member of the basal transcription complex implicated in p53-mediated transcription, is synthesized as several alternately spliced variants. The predominant forms found in normal and neoplastic breast epithelial cells are shown to be 72 kDa (TAF_II70) and 78 kDa (TAF_II80). Most cancers express higher levels of the TAF_II80 isoform, whereas normal breast epithelia express higher levels of the TAF_II70 isoform. Expression of TAF_II70, but not TAF_II80, causes dramatic growth suppression of normal and transformed breast epithelial cell lines in a p53-independent manner. Growth suppression correlates with mitotic inhibition resulting from an increased number of cells in G₂. Both isoforms induce expression of the G₂ arrest associated gene, GADD45a, but a novel protein-protein interaction was observed between TAF_II70 (not TAF_II80) and GADD45a, suggesting that this interaction is important for the observed growth arrest phenotype induced by the TAF_II70 isoform. GADD45a null cells are not subject to TAF_II70 inhibition, further supporting the relevance of this interaction.

This article has been cited by other articles:

				J. A. Calarco, Y. Xing, M. Caceres, J. P. Calarco, X. Xiao, Q. Pan, C. Lee, T. M. Preuss, and B. J. Blencowe Global analysis of alternative splicing differences between humans and chimpanzees Genes & Dev., November 15, 2007; 21(22): 2963 - 2975. [Abstract] [Full Text] [PDF]

				Q. Chang, D. Bhatia, Y. Zhang, T. Meighan, V. Castranova, X. Shi, and F. Chen Incorporation of an Internal Ribosome Entry Site-Dependent Mechanism in Arsenic-Induced GADD45{alpha} Expression Cancer Res., July 1, 2007; 67(13): 6146 - 6154. [Abstract] [Full Text] [PDF]

				Y. Zhang, D. Bhatia, H. Xia, V. Castranova, X. Shi, and F. Chen Nucleolin links to arsenic-induced stabilization of GADD45{alpha} mRNA Nucleic Acids Res., January 18, 2006; 34(2): 485 - 495. [Abstract] [Full Text] [PDF]